Bacterial Self-Healing Concrete: Mechanisms, Incorporation Methods, and Future Perspectives — A Comprehensive Review

Ruqaya F. Hamada; Enas Muhi Hadi; Shihab Ahmed Zaidan

doi:10.71229/p8ydfx13

Authors

Ruqaya F. Hamada Department of Medical and Industrial Material Science, College of Applied Science, University of Technology, Al-Sina’a Street, 10066 Baghdad, Iraq https://orcid.org/0009-0005-7407-9588
Enas Muhi Hadi Department of Medical and Industrial Material Science, College of Applied Science, University of Technology, Baghdad 10066, Iraq
Shihab Ahmed Zaidan Department of Medical and Industrial Material Science, College of Applied Science, University of Technology, Baghdad 10066, Iraq

DOI:

https://doi.org/10.71229/p8ydfx13

Keywords:

Bio -concrete, Self -healing concrete , Calcium carbonate precipitation , Bacillus spp, Biomineralization , Sustainable construction

Abstract

Bio-concrete or self-healing concrete is an innovative concept concerning sustainable construction materials. Contrary to regular concrete prone to progressive deterioration due to the formation of cracks, specific types of alkali-tolerant bacteria, primarily Bacillus, make it possible for bio-concrete to produce CaCO₃ precipitation in emerging cracks so that autonomous restoration of integrity occurs through healing without any direct participation. The rising popularity of such biotechnology in the construction industry should be accounted for by its capacity to reduce the costs associated with maintenance and enhance the longevity of concrete structures. This literature review evaluates the evolution of the idea of bio-concrete starting from the theoretical background laid out in the early 1990s till today's application. The focus is on the principles of biomineralization, the process of production, and characteristics of dominant strains. Particular attention is drawn to advantages, in terms of enhanced compressive strength up to 40%, and limitations, including higher costs of production and challenges concerning bacteriology, of utilizing this type of concrete. At last, knowledge gaps in this sphere are indicated, and priorities in further research are set concerning the following issues: standardization, testing under extreme conditions, life cycle assessment, and applying machine learning.

References

[1] S. Sangadji, “Can self-healing mechanism help concrete structures sustainable?” Procedia Engineering, vol. 171, pp. 238–249, 2017.

[2] S. Dinesh, R. Shanmugapriyan, and S. N. Sheen, “A review on bacteria-based self-healing concrete,” Imperial Journal of Interdisciplinary Research, vol. 3, no. 1, pp. 1023–1026, 2017.

[3] B. R. Gautam, “Bacteria-based self-healing concrete — a bacterial approach,” International Journal of Engineering and Science, pp. 57–61, 2018.

[4] B. Aytekin, A. Mardani, and Ş. Yazıcı, “State-of-art review of bacteria-based self-healing concrete,” Construction and Building Materials, vol. 378, p. 131198, 2023.

[5] Y. Yang et al., “Autogenous healing of engineered cementitious composites under wet–dry cycles,” Cement and Concrete Research, vol. 39, no. 5, pp. 382–390, 2009.

[6] S. Yang et al., “A review on cementitious self-healing and the potential of phase-field methods,” Materials, vol. 13, 2020.

[7] A. Algaifi et al., “Insight into the role of microbial calcium carbonate and the factors involved in self-healing concrete,” Construction and Building Materials, vol. 254, p. 119258, 2020.

[8] N. Nain et al., “Enhancement in strength parameters of concrete by application of Bacillus bacteria,” Construction and Building Materials, vol. 202, pp. 904–908, 2019.

[9] Y. Su et al., “Influence of bacterial self-healing agent on early-age performance of cement-based materials,” Construction and Building Materials, vol. 218, pp. 224–234, 2019.

[10] F. Cappitelli et al., “Advantages of using microbial technology over conventional chemical technology in removal of black crusts from stone surfaces,” Appl. Environ. Microbiol., vol. 73, no. 17, pp. 5671–5675, 2007.

[11] A. Barberan et al., “A new area of application and research in bioprocesses: biotechnologies in civil construction,” Revista Bionatura, vol. 5, no. 1, pp. 1072–1077, 2020.

[12] S. K. Ramachandran, V. Ramakrishnan, and S. S. Bang, “Remediation of concrete using micro-organisms,” ACI Materials Journal, vol. 98, no. 1, pp. 3–9, 2001.

[13] S. Deb, “Self-healing concrete,” The Masterbuilder, 2012.

[14] I. M. Elgendy et al., “Bacteria-powered self-healing concrete: breakthroughs, challenges, and future prospects,” J. Ind. Microbiol. Biotechnol., vol. 52, no. 4, 2025.

[15] P. Kalita and H. Tag, “Development and enhancement of self-healing bacterial concrete and its application as sustainable concrete in modern construction,” Geomicrobiology Journal, vol. 42, pp. 1076–1100, 2025.

[16] W. De Muynck, N. De Belie, and W. Verstraete, “Microbial carbonate precipitation in construction materials: a review,” Ecological Engineering, vol. 36, no. 2, pp. 118–136, 2010.

[17] N. K. Dhami, M. S. Reddy, and A. Mukherjee, “Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites,” Journal of Microbiology and Biotechnology, vol. 23, no. 5, pp. 707–714, 2013.

[18] V. Achal and A. Mukherjee, “A review of microbial precipitation for sustainable construction,” Construction and Building Materials, vol. 93, pp. 1224–1235, 2015.

[19] M. F. S. Zawad, M. A. Rahman, and S. N. Priyom, “Bio-engineered concrete: a critical review on the next generation of durable concrete,” Journal of the Civil Engineering Forum, vol. 7, no. 3, pp. 335–358, 2021.

[20] Giatec Scientific Inc., “Self-healing bio-concrete,” 2023.

[21] A. Talaiekhozani et al., “A review of self-healing concrete research development,” Journal of Environmental Treatment Techniques, vol. 2, no. 1, pp. 1–11, 2013.

[22] J. Xu, X. Wang, and B. Wang, “Biochemical process of ureolysis-based microbial CaCO₃ precipitation and its application in self-healing concrete,” Appl. Microbiol. Biotechnol., vol. 102, no. 7, pp. 3121–3132, 2018.

[23] S. Jayanthi et al., “Assessment of bacteria-based self-healing concrete through experimental investigations,” J. Mater. Sci.: Mater. Eng., vol. 20, art. 15, 2025.

[24] Q. Qtiashat et al., “Influence of Bacillus subtilis bacteria on strength and durability of concrete with silica fume,” Civil Engineering Journal, vol. 11, no. 5, pp. 1959–1968, 2025.

[25] D. Aceituno-Caicedo et al., “Impact of bacterial admixtures on the compressive and tensile strengths, permeability, and pore structure of ternary mortars,” Biotechnology Journal, vol. 19, no. 1, 2024.

[26] H. M. Jonkers and E. Schlangen, “Development of a bacteria-based self-healing concrete,” in Tailor Made Concrete Structures, Taylor & Francis, 2008, pp. 425–430.

[27] R. Siddique and N. K. Chahal, “Effect of ureolytic bacteria on concrete properties,” Constr. Build. Mater., vol. 25, pp. 3791–3801, 2011.

[28] K. Van Tittelboom et al., “Use of bacteria to repair cracks in concrete,” Cem. Concr. Res., vol. 40, pp. 157–166, 2010.

[29] N. De Belie et al., “A review of self-healing concrete for damage management of structures,” Adv. Mater. Interfaces, vol. 5, no. 17, 2018.

[30] B. B. Mitikie and W. A. Elsaigh, “Bio-based bacterial concrete: a sustainable alternative in construction,” Innov. Infrastruct. Solut., vol. 10, art. 234, 2025.

[31] R. Pei et al., “Use of bacterial cell walls to improve the mechanical performance of concrete,” Cem. Concr. Compos., vol. 39, pp. 122–130, 2013.

[32] H. M. Jonkers et al., “Application of bacteria as self-healing agent for the development of sustainable concrete,” Ecol. Eng., vol. 36, pp. 230–235, 2010.

[33] S. Soundharya and K. Nirmalkumar, “Study on the effect of calcite-precipitating bacteria on self-healing mechanism of concrete,” Int. J. Eng. Res. Manag. Technol., vol. 1, no. 4, pp. 202–208, 2014.

[34] S. Qian, J. Zhou, and E. Schlangen, “Influence of curing condition and pre-cracking time on the self-healing behaviour of engineered cementitious composites,” Cem. Concr. Compos., vol. 32, no. 9, pp. 686–693, 2010.

[35] Y. Ç. Erşan et al., “Screening of bacteria and concrete-compatible protection materials,” Constr. Build. Mater., vol. 88, pp. 196–203, 2015.

[36] V. Wiktor and H. M. Jonkers, “Quantification of crack-healing in novel bacteria-based self-healing concrete,” Cem. Concr. Compos., vol. 33, pp. 763–770, 2011.

[37] N. K. Dhami, M. S. Reddy, and A. Mukherjee, “Improvement in strength properties of ash bricks by bacterial calcite,” Ecol. Eng., vol. 39, pp. 31–35, 2012.

[38] V. Achal, X. Pan, and D. Kumari, “Improved strength and durability of fly-ash-amended concrete by microbial calcite precipitation,” Ecol. Eng., vol. 37, pp. 554–559, 2011.

[39] N. K. Dhami, S. M. Reddy, and A. Mukherjee, “Biofilm and microbial applications in biomineralized concrete,” in Advances in Biomineralization, 2012.

[40] A. M. Metwally, M. Mahdy, and H. El-Raheem, “Performance of bio-concrete using Bacillus pasteurii bacteria,” J. Civ. Eng., vol. 6, no. 8, 2020.

[41] H. Wu et al., “Effect of crystalline admixture and superabsorbent polymer on self-healing and mechanical properties of mortar,” Materials, vol. 15, no. 17, p. 6040, 2022.

[42] S. J. Park et al., “Calcite-forming bacteria for compressive-strength improvement in mortar,” J. Microbiol. Biotechnol., vol. 20, no. 4, pp. 782–788, 2010.

[43] T. H. Nguyen et al., “Bacterial self-healing of concrete and durability assessment,” Cem. Concr. Compos., vol. 104, 2019.

[44] J. Y. Wang et al., “Self-healing concrete by use of microencapsulated bacterial spores,” Cem. Concr. Res., vol. 56, pp. 139–152, 2014.

[45] J. Y. Wang et al., “Application of hydrogel-encapsulated carbonate-precipitating bacteria for approaching a realistic self-healing in concrete,” Constr. Build. Mater., vol. 68, pp. 110–119, 2014.

[46] Y. Li et al., “Preparation and application of novel microcapsules ruptured by microwave for self-healing concrete,” Constr. Build. Mater., vol. 304, p. 124616, 2021.

[47] M. E. Espitia-Nery et al., “Mechanisms of encapsulation of bacteria in self-healing concrete,” Dyna, vol. 86, no. 210, pp. 17–22, 2019.

[48] S. Sangadji and E. Schlangen, “Mimicking the bone-healing process to self-repair concrete structures,” Procedia Engineering, vol. 54, pp. 315–326, 2013.

[49] K. S. Toohey et al., “Self-healing materials with microvascular networks,” Nature Materials, vol. 6, no. 8, pp. 581–585, 2007.

[50] B. Dong et al., “A microcapsule-technology-based self-healing system for concrete structures,” Journal of Earthquake and Tsunami, vol. 7, no. 3, 2013.

[51] L. V. Zhang et al., “Crack self-healing in bio-green concrete,” Composites Part B: Engineering, vol. 227, p. 109397, 2021.

[52] J. Wang et al., “Use of silica gel or polyurethane-immobilised bacteria for self-healing concrete,” Constr. Build. Mater., vol. 26, pp. 532–540, 2012.

[53] A. Elmoaty, “Self-healing of polymer-modified concrete,” Alexandria Engineering Journal, vol. 50, no. 2, pp. 171–178, 2011.

[54] J. Hanna, “Self-healing concrete techniques, technologies and applications,” Recent Progress in Materials, vol. 6, no. 1, 2024.

[55] D. Snoeck et al., “Self-healing cementitious materials by the combination of microfibres and superabsorbent polymers,” J. Intell. Mater. Syst. Struct., vol. 25, no. 1, pp. 13–24, 2014.

[56] P. Y. Wong, “Advances in microbial self-healing concrete: a critical review of mechanisms, developments, and future directions,” Science of the Total Environment, vol. 947, p. 174553, 2024.

[57] A. Vedrtnam et al., “Ranking bacteria for carbon capture and self-healing in concrete,” Sustainability, vol. 17, no. 12, p. 5353, 2025.

[58] S. Shahid et al., “Self-healing of cracks in concrete using Bacillus strains encapsulated in sodium-alginate beads,” ChemistrySelect, vol. 5, no. 1, pp. 312–323, 2020.

[59] A. Ivaškė et al., “Bacterial viability in self-healing concrete: a case study of non-ureolytic Bacillus species,” Microorganisms, vol. 11, no. 10, p. 2402, 2023.

[60] A. Thakur, A. Phogat, and K. Singh, “Bacterial concrete and effect of different bacteria on the strength and water-absorption characteristics of concrete: a review,” Int. J. Civ. Eng. Technol., vol. 7, no. 5, pp. 43–56, 2016.

[61] A. Talaiekhozani et al., “Application of Proteus mirabilis and Proteus vulgaris mixture to design self-healing concrete,” Desalination and Water Treatment, vol. 52, pp. 3623–3630, 2014.

[62] C. Hadhinata et al., “Multi-perspective review on application of self-healing concrete and its prospect in Asia,” AIP Conference Proceedings, 2022.

[63] R. P. Borg, “Water-tower conservation project at the Public Abattoir in Marsa (Malta),” Horizon Magazine, European Commission, 2022.

[64] M. G. Beltran et al., “Field application of self-healing concrete with natural fibres as linings for irrigation canals in Ecuador,” 2015.

[65] A. Vedrtnam, K. Kalauni, and M. T. Palou, “Validated thermal model for bacterial survival in fire-resistant self-healing concrete,” Scientific Reports, 2025.

[66] X. Huang et al., “Self-healing performance assessment of bacterial-based concrete using machine-learning approaches,” Materials, vol. 15, no. 13, p. 4436, 2022.

[67] F. S. de Oliveira and R. Stefani, “Evaluating the use of synthetic data for machine-learning prediction of the self-healing capacity of concrete,” AI in Civil Engineering, vol. 4, art. 25, 2025.

[68] X. Jin et al., “Sustainability-oriented concrete LCA: an integrated performance-cost-durability framework,” Construction and Building Materials, 2025.

[69] Buildings, “Effect of a bacterial consortium on the mechanical and durability properties of self-healing concrete at different water-cement ratios,” Buildings, vol. 16, no. 10, p. 1997, 2026.

[70] A. Van Wylick et al., “A review on the potential of filamentous fungi for microbial self-healing of concrete,” Fungal Biology and Biotechnology, 2021.