Effect of DBD cold Plasma on Anti-bacterial and Biofilm Removal of (E. coli and E. faecalis)

dhrgham55 qadsyiah; Abdulhussain A.

doi:10.71229/6b4grs31

Authors

Dhurgham Mohamed Ali Department of Physics, College of Education, University of Al-Qadisiyah, Al-Diwaniyah, Al-Qadisiyah, Iraq.
Abdulhussain A.Khadayeir Department of Physics, College of Education, University of Al-Qadisiyah, Al-Diwaniyah, Al-Qadisiyah, Iraq.

DOI:

https://doi.org/10.71229/6b4grs31

Keywords:

Cold atmospheric plasma (CAP) , Dielectric barrier discharge (DBD), Antibacterial activity; , Escherichia coli , Enterococcus faecalis

Abstract

In this work, an atmospheric-pressure argon DBD plasma jet was Applied at antibacterial activity on clinically isolated Gram-positive and Gram-negative bacteria was investigated. The plasma system was operated at voltages between 10 and 16 kV and argon flow rates of 1–6 L min⁻¹. OES analysis identified the reactive species produced in plasma discharge that contribute to the bacterial inactivation. Plasma treatment significantly reduced the bacterial cell density and the inactivation efficiency increased with the increase of the exposure time. SEM observations showed that the bacterial cells were severely damaged after plasma exposure, indicating the cell membrane disruption. The results suggest that the developed argon DBD plasma system is an efficient and environment-friendly method for bacterial decontamination and it has great potential to be used in biomedical sterilization and surface disinfection.

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